nounIntermediate
Definition
The gold standard analytical technique for measuring peptide purity.
Detailed Explanation
The gold standard analytical technique for measuring peptide purity. A peptide sample dissolved in mobile phase is pumped through a column packed with stationary phase particles. Different components separate based on their affinity for the stationary phase and elute at different retention times, detected by UV absorbance at 214 nm (peptide bond absorption). Reversed-phase HPLC (RP-HPLC) using C18 columns is the most common mode for peptides. A Certificate of Analysis (COA) for a commercial peptide should always report HPLC purity — typically ≥95% for research grade and ≥98% for pharmaceutical/compounding grade.
Key Facts
- The gold standard analytical technique for measuring peptide purity.
- A peptide sample dissolved in mobile phase is pumped through a column packed with stationary phase particles.
- Different components separate based on their affinity for the stationary phase and elute at different retention times, detected by UV absorbance at 214 nm (peptide bond absorption).
- Reversed-phase HPLC (RP-HPLC) using C18 columns is the most common mode for peptides.
- A Certificate of Analysis (COA) for a commercial peptide should always report HPLC purity — typically ≥95% for research grade and ≥98% for pharmaceutical/compounding grade.
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