Detailed Explanation
Analytical technique that measures the mass-to-charge ratio (m/z) of ions to identify, quantify, and structurally characterize peptides. In peptide science, liquid chromatography–mass spectrometry (LC-MS) is the gold standard for quality control, purity assessment, and sequence confirmation.
Tandem MS (MS/MS) fragments peptides into characteristic b-ions and y-ions that reveal the amino acid sequence. Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) are the two primary ionization methods. MS is reported alongside HPLC on peptide Certificates of Analysis to confirm identity (correct molecular weight) and purity.
Key Facts
- Analytical technique that measures the mass-to-charge ratio (m/z) of ions to identify, quantify, and structurally characterize peptides.
- In peptide science, liquid chromatography–mass spectrometry (LC-MS) is the gold standard for quality control, purity assessment, and sequence confirmation.
- Tandem MS (MS/MS) fragments peptides into characteristic b-ions and y-ions that reveal the amino acid sequence.
- Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) are the two primary ionization methods.
- MS is reported alongside HPLC on peptide Certificates of Analysis to confirm identity (correct molecular weight) and purity.
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